Part:BBa_K081001

Mux CH1

This device is a PoPS in -> lasI out protein generator with Plas promoter downstream.

It is a module of the following genetic multiplexer (mux - click [http://en.wikipedia.org/wiki/Multiplexer here] to see what is a multiplexer):

where:

• PA, PS and PB are three promoters that can be ACTIVATED by the three generic elements A, S and B respectively;
• GOI is the gene of interest that is the output of the system.

In particular, K081001 is Channel 1.

People who want to use the genetic mux have to assemble:

• K081000 under the desired regulation for Channel 0 (for example the generic promoter PA);
• K081001 under the desired regulation for Channel 1 (for example the generic promoter PB);
• K081002 under the desired regulation for Selector (for example the generic promoter PS);
• the desired protein generator downstream of K081000 and K081001 (for example the generic RBS-GOI-T generator);

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 801
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 682
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 244
  
• 1000
  COMPATIBLE WITH RFC[1000]